Experimental Biology and Medicine > Volume 234, Number 4 > Pp. 403-409

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ORIGINAL RESEARCH ARTICLE

Mucosal Immunization with Polyamine Transport Protein D (PotD) Protects Mice Against Nasopharyngeal Colonization with Streptococcus pneumoniae

Pratik Shah*, David E. Briles, Janice King, Yvette Hale and Edwin Swiatlo*||1

^* Department of Microbiology, University of Mississippi Medical Center, Jackson, Mississippi 39216; Department of Microbiology, University of Alabama at Birmingham, Birmingham, Alabama 35294; Department of Pediatrics, University of Alabama at Birmingham, Birmingham, Alabama 35233; Department of Medicine, University of Mississippi Medical Center, Jackson, Mississippi 39216; and ^|| Research Service, Veterans Affairs Medical Center, Jackson, Mississippi 39216

¹ Research Service (151), Veterans Affairs Medical Center, 1500 Woodrow Wilson Drive, Jackson, MS 39216. E-mail: edwin.swiatlo{at}va.gov

Streptococcus pneumoniae is an encapsulated pathogen that can cause invasive disease following colonization of the nasopharynx. Targeting colonization of mucosal surfaces may, therefore, be the best approach for vaccination to prevent pneumococcal invasive disease. Previous studies in our laboratory have shown that immunization with recombinant polyamine transport protein D (PotD) protects mice against systemic pneumococcal infections. In this study we investigated the efficacy of mucosal immunization with rPotD to protect against pneumococcal carriage and invasion in a murine model. Mice were intranasally immunized with either rPotD and cholera toxin B subunit (CTB) or CTB alone. Significantly less pneumococci were recovered from the nasopharynx of immunized mice compared to the control animals following intranasal challenge with either EF3030 (serotype 19F) (P < 0.05) or an invasive serotype 4 isolate (TIGR4) (P < 0.05). PotD immunized mice also had lesser bacteria in their sinus tissues (P < 0.05), brains (P < 0.05), lungs and olfactory bulbs following intranasal challenge with TIGR4. ELISA analysis demonstrated the presence of IgG antibodies to PotD in the serum and IgA antibodies in the saliva. These results indicate that mucosal immunization with PotD generates both mucosal and systemic immune responses and prevents establishment of nasopharyngeal carriage by multiple pneumococcal serotypes. Thus, PotD is a potentially important antigen for development of a pneumococcal protein vaccine.

Keywords: Streptococcus pneumoniae , PotD, vaccine

The authors declare no conflict of interest.

Presented at the 108th American Society for Microbiology general meeting on June 4, 2008 in Boston, MA.

This research was funded by the Veterans Administration Office of Research.

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